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Image Search Results
Journal: Oncotarget
Article Title: Interleukin-34 sustains pro-tumorigenic signals in colon cancer tissue
doi: 10.18632/oncotarget.23289
Figure Lengend Snippet: ( A ) Paired colonic explants taken from non-tumoral (NT) and tumoral (T) areas of 12 patients with sporadic CRC were analyzed for IL-34 RNA expression by real-time PCR and levels were normalized to β-actin. Each point represents the value of IL-34 mRNA in a single patient and horizontal bars indicate the median value. ( B ) Representative Western blots showing IL-34 and β-actin in total proteins extracted from paired non-tumoral (NT) and tumoral (T) areas of two patients with sporadic CRC. Bottom panels show the quantitative analysis of IL-34/β-actin ratio in samples taken from paired non-tumoral (NT) and tumoral (T) areas of 5 CRC patients as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate mean ± SEM of all samples. ( C ) Quantitative analysis of IL-34 protein expression in paired colonic explants taken from the non-tumoral (NT) and tumoral (T) areas of 8 patients with sporadic CRC were measured by ELISA. Data were expressed as mean ± SEM of all samples. ( D ) Representative photomicrographs (100× original magnification) of IL-34-stained frozen sections of surgical specimens taken from non-tumoral (NT) and tumoral (T) areas of one patient with sporadic CRC. Staining with isotype control antibody is also shown. Right panel shows the number of IL-34-positive cells for high power field (hpf) of 8 separate experiments in which sections of 8 CRC patients were analyzed. Data are expressed as mean ± SEM.
Article Snippet:
Techniques: RNA Expression, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Staining, Control
Journal: Oncotarget
Article Title: Interleukin-34 sustains pro-tumorigenic signals in colon cancer tissue
doi: 10.18632/oncotarget.23289
Figure Lengend Snippet: ( A ) Representative Western blots showing M-CSFR-1, PTP-z and β-actin in total proteins extracted from paired non-tumoral (NT) and tumoral (T) areas of 2 patients with sporadic CRC. Right panels show the quantitative analysis of M-CSFR-1/β-actin and PTP-z/β-actin ratio respectively in paired non-tumoral (NT) and tumoral (T) areas taken from 5 CRC patients as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate mean ± SEM of all samples. ( B ) Representative photomicrographs (100x original magnification) of M-CSFR-1-and PTP-z stained paraffin-embedded sections of surgical colonic samples taken from one control (CTR) and one patient with sporadic CRC. The figure is representative of 3 separate experiments in which sections taken from 4 CTR and 3 patients with CRC were analyzed for M-CSFR-1 and PTP-z. Staining with isotype control antibody is also shown.
Article Snippet:
Techniques: Western Blot, Staining, Control